Reconstitution, Storage & Handling Guide

1. 1

   Equilibrate to room temperature

   Remove the lyophilized vial from cold storage and allow it to reach room temperature. Reconstituting a cold vial can stress the peptide and slow dissolution.

2. 2

   Sanitize both stoppers

   Wipe the rubber stopper of the peptide vial and the bacteriostatic water (BAC) vial with a fresh alcohol swab and let them air-dry before any needle contact.

3. 3

   Draw the diluent

   Using a sterile syringe, draw the chosen volume of bacteriostatic water. See the mixing table below to convert your target reconstitution volume into concentration per unit.

4. 4

   Add diluent down the vial wall

   Insert the needle at an angle and let the water run slowly down the inner glass wall — never inject the stream directly onto the lyophilized powder, which can denature the peptide.

5. 5

   Dissolve gently — do not shake

   Swirl or roll the vial gently between your fingers until fully clear. Vigorous shaking introduces shear stress and foaming. If particulate or cloudiness remains, do not use.

6. 6

   Refrigerate after reconstitution

   Once in solution, store the vial at 2–8 °C (refrigerated). Reconstituted peptides are far less stable than the lyophilized form and should be used within the typical research window below.

Resulting concentration per 10 units (0.1 mL) on a standard U-100 insulin syringe. Use the live Protocol Calculator for exact per-dose volumes.

Lyophilized vials store long-term frozen; reconstituted solutions are refrigerated and have a shorter usable research window. Ranges below are typical literature values — always defer to the product's stated storage conditions.